Ripemedia sent me his microphone, and the root cause of the current issues is very interesting, so posting here for posterity.
He could not trim more than about 1mA of bias current out of the 12AT7, which I had diagnosed as either a leaking cap (like previous
builders had experienced), but the root cause: grid leak biasing. According to RDH4, grid leak (or contact potential) bias is most
pronounced when the cathode and grid are near the same potential: hence, if the cathode and grid approach within 1V of each other,
then grid leakage increases.
In this design, cathode is grounded, and grid sits at -1V. However with a 12AT7, being much higher mu than a 6072A, the grid leakage
current is much higher. Hence, when you try to trim the bias closer to ground than about -1V, grid leakage starts to rise, which
in effect cancels the bias from the supply trying to go more positive. This causes the bias to be 'clamped' at -1V, regardless of
how high the bias voltage is in the PSU.
I couldn't see it clearly until I wired up a TL071 in a unity gain follower configuration, powered by two, 9V batteries. The input
bias current of this FET opamp is typically on the order of a handful of pA, meaning that when measuring close to 0V the input impedance
can approach north of several tera-ohm. This is high enough to be able to probe the >250Mohm nodes with reasonable accuracy.
In fact, there are special purpose capacitive sensor op-amps than can be used which have even higher input impedances.
In any case, starting from -5V, and dialing down the bias in the PSU toward 0V, you can clearly see the bias at the gate of the tube
lower, then stop lowering at about -1.26V. This means that with as 12AT7 tube, you can set the bias voltage to 0V (dial the pot to minimum)
and the tube will happily idle at the correct -1V bias.
. The left DVM is showing the PSU bias point, and the right DVM is reading the output of the op-amp which is measuring the grid of the tube. At -3.5V, we get -3.5V at the tube, however when set to 0V, you can see the tube is still seeing about -1.1V.
I had not seen this on the 6072A, as being medium-ish mu the effect is far less pronounced since the plate-to-cathode current is so much less.